Protein A Affinity Chromatography Application

Protein A affinity chromatography medium by EPRUI is based on the monodisperse porous poly (methyl methacrylate) (PMMA) microspheres as the matrix, and genetically modified protein A as the ligand, which ensures its high affinity binding of monoclonal antibodies and Fc fragment containing recombinant proteins (macromolecules).

Our protein A chromatography media has high mechanical strength and good pH stability. Even at a high flow rate, the beads still maintained a high dynamic binding capacity, meeting the demands from laboratory preparation to industrial production.

Protein A Affinity Chromatography Application

Applications Instructions

  1. Replace 20% ethanol preservation solution with pure water before use
  2. Rinse and equilibrate the column with eluent, such as 100 mM Gly, pH 3.0 and equilibration solution, such as 20 mM PBS, 150 mM NaCl, pH 7.0
  3. Injection,wash with 10 CV balance solution until baseline balance
  4. Elution, wash with 15 CV eluent until baseline balance
  5. Rebalance,wash with 15 CV balance solution until baseline balance
  6. After using,first use pure water to replace the buffer salt in column, and then store in 20% ethanol.

Note: During the analysis process, both the sample and the mobile phase must be filtered with a membrane with pore size of 0.45 μm.

Example: Antibody capture in horse plasma samples

Protein A affinity chromatography has great performance when capture antibody in horse plasma samples.

Protein A Affinity Chromatography Application

Binding Buffer: 20 mM PB, 150 mM NaCl, pH 7.0; Elution Buffer: 100 mM Gly, pH 3.0.